Novaferon, a novel recombinant protein produced by DNA-shuffling of IFN-α, shows antitumor effect in vitro and in vivo
摘要：
Objective A recombinant antitumor/antiviral protein (Novaferon, Nova) is a new type of interferon, which is produced by artificial design technology combining DNA-shuffling and High Throughput Screening (HTS). Methods The in vitro biological activities, such as anti-tumor activity and antiviral activity of Nova and recombinant human interferon alpha-2b (rhIFN-??2b) was performed; in vivo anti-tumor activity in nude mice was also tested. Flow cytometry, histo-pathological analysis including HE staining and immunohistochemistry, and surface plasmon resonance assay were performed to investigate the underlying mechanisms analysis. Results Nova exhibited stronger anti-cancer effects compared to rhIFN-??2b in vitro and in vivo. The antitumor mechanisms of Nova may be related to S phase arrest, pro-apoptosis, and inhibition of tumor angiogenesis. Moreover, Nova exhibited a higher binding affinity for IFN receptor 2 (IFNR2) than rhIFN-??2b, which is one of the possible reasons accounting for its stronger actions against tumor cells compared with rhIFN-??2b. Conclusion Nova has strong antitumor activity and could be a potentially effective therapeutic drug for cancer.
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关键词：
Recombinant interferon-?? protein Anti-cancer Novaferon IFN receptor 2 in vivo Internal Medicine Medicine(General Oncology
DOI：
10.1186/1475-2867-14-8
被引量：
年份：
2014
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