Identification of a polypeptide encoded by the avian sarcoma virus src gene
摘要：
Two techniques were used to search for the polypeptide encoded by the avian sarcoma virus (ASV) src gene. First, antiserum from rabbits bearing ASV-induced fibrosarcomas was used to immunoprecipitate a transformation-specific antigen from ASV-transformed chick embryo fibroblasts. This antigen has an apparent molecular weight (Mr) of 60,000. Second, the 3′one-third of the ASV genome, selected by oligo(dT)-cellulose chromatography and sucrose gradient sedimentation, was translated in a mRNA-dependent reticulocyte cell-free lysate. This RNA species programmed the synthesis of a polypeptide that comigrated with the transformation-specific antigen of Mr60,000 immunoprecipitated from transformed cells. The methionine-containing tryptic peptides from the polypeptides of Mr60,000 obtained from translation in vitro and from immunoprecipitation were found to be identical upon two-dimensional fractionation.
展开
关键词：
Microbiology Cell-free protein synthesis Translation of viral RNA Immunoprecipitation Peptide fingerprints
DOI：
10.1073/pnas.75.3.1567
被引量：
年份：
1978
Semantic Scholar
掌桥科研
万方医学
dx.doi.org
NCBI
查看更多
Europe PMC
JSTOR
NCBI
Europe PMC
(全网免费下载)
NCBI
(全网免费下载)
adsabs.harvard.edu
ResearchGate
EBSCO
onAcademic
web.stanford.edu
(全网免费下载)
pnas.org
(全网免费下载)
mysciencework.com
pnas.org
deepdyve.com
mendeley.com
core.ac.uk
jvi.asm.org
The National Academy of Sciences of the United States of America
(全网免费下载)
NCBI
(全网免费下载)
Europe PMC
(全网免费下载)
web.stanford.edu
(全网免费下载)
pnas.org
(全网免费下载)
The National Academy of Sciences of the United States of America
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
引用走势
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
