Drosophila ventral furrow morphogenesis: a proteomic analysis.

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摘要：

Ventral furrow formation is a key morphogenetic event duringDrosophila gastrulation that leads to the internalization ofmesodermal precursors. While genetic analysis has revealed the genes involvedin the specification of ventral furrow cells, few of the structural proteinsthat act as mediators of ventral cell behavior have been identified. Acomparative proteomics approach employing difference gel electrophoresis wasused to identify more than fifty proteins with altered abundance levels orisoform changes in ventralized versus lateralized embryos. Curiously, themajority of protein differences between these embryos appeared well beforegastrulation, only a few protein changes coincided with gastrulation,suggesting that the ventral cells are primed for cell shape change. Threeproteasome subunits were found to differ between ventralized and lateralizedembryos. RNAi knockdown of these proteasome subunits and time-dependentdifference-proteins caused ventral furrow defects, validating the role ofthese proteins in ventral furrow morphogenesis.

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关键词：

Drosophila gastrulation ventral furrow formation proteomics

DOI：

10.1242/dev.00955

被引量：

120

年份：

2004