Receptor assay-guided isolation of anti-GABAergic insecticidal alkaloids from a fungal culture
摘要：
The γ-aminobutyric acid (GABA) receptor bears sites of action for insecticides. To discover GABA receptor-directed insecticides in natural products, fungal culture extracts were screened for their ability to inhibit specific binding of the radiolabeled noncompetitive antagonist [3H]1-(4-ethynylphenyl)-4-n-propyl-2,6,7-trioxabicyclo[2.2.2]octane to housefly head membranes. The screening efforts led to the isolation of two alkaloids from Aspergillus terreus: PF1198A (alantrypinone) and PF1198B (serantrypinone), which had IC50 values of 0.34 and 2.1 μM, respectively, in this assay. These compounds were ca. 4761-fold selective for housefly vs rat GABA receptors. Both compounds showed insecticidal activity against Myzus persicae in the range of 100500 ppm. Binding assay-guided screening should provide significant opportunities for the identification of novel and selective insecticides. Keywords: GABA receptor; insecticidal compounds; PF1198; alantrypinone; serantrypinone
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DOI：
10.1021/jf049870g
被引量：
年份：
2004
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