摘要：

AIM:To evaluate the effects of p53 on apoptosis andproliferation of hepatocellular carcinoma (HCC) cells treatedwith transcatheter arterial chemoembolization (TACE).METHODS:A total of 136 patients with HCC received TACEand other management before surgery were divided intoTACE group and non-TACE group.TACE group included 79patients who had 1-5 courses of TACE before surgery,ofthem,11 patients had 1-4 courses of chemotherapy (groupA),33 patients had 1-5 courses of chemotherapy combinedwith iodized oil (group B),23 patients had 1-3 courses ofchemotherapy,iodized oil and gelatin sponge (group C),12patients had 1-3 courses of chemotherapy combined withiodized oil,ethanol and gelatin sponge (group D).Non-TACEgroup included the remaining 57 patients who had surgeryonly.The extent of apoptosis was analyzed by transferasemediated dUTP nick end labeling (TUNEL) staining.Theexpressions of p53,Bcl-2,Bax,Ki-67 and PCNA protein weredetected by immunohistochemical method.RESULTS:P53 protein expressions in trabecular and clearcells in HCC specimens were significantly lower than that inpseudoglandar,solid,poorly differentiated or undifferentiatedand sclerosis HCC (P<0.05).Expression of p53 protein inHCC cells increased with the increase of pathological grades(P<0.05),and correlated positively with expressions of Ki-67 and PCNA protein,and negatively with Bcl-2 to Bax proteinexpression rate and AI (P<0.05).Expression of p53 proteinwas significantly higher in group A than in groups B,C,Dand the non-TACE group,and was higher in group B than ingroups C and D,and lower in group D than in the non-TACEgroup (P<0.05).CONCLUSION:Expression of p53 protein can enhanceproliferation of HCC cells and suppress apoptosis of HCCcells after TACE.

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