Inhibition of membrane phosphotyrosyl-protein phosphatase activity by vanadate

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摘要：

We have investigated the effect of vanadate on the phosphoserine- and phosphotyrosine-specific phosphoprotein phosphatase activities of A-431 cell membranes and have found that micromolar concentrations of vanadate strongly inhibit the membrane-dependent dephosphorylation of histones containing phosphotyrosine but that they do not inhibit the dephosphorylation of histones containing phosphoserine and phosphothreonine. In addition, the dephosphorylation of endogeneous membrane proteins of A-431 cells (which are known to be phosphorylated at tyrosine residues) was inhibited by vanadate. These results show that vanadate is a potent and selective inhibitor of phosphotyrosyl-protein phosphatase.

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关键词：

EGF epidermal growth factor epidermal growth factor phospho-Tyr-histones histones phosphorylated at tyrosine histones phosphorylated at tyrosine phospho-Ser-histones histones phosphorylated at serine and threonine histones phosphorylated at serine and threonine

DOI：

10.1016/0006-291X(82)90635-0

被引量：

1165

年份：

1982