摘要：

Colloidal biocatalysts, comprising polystyrene (PS) carrier particles coated with enzyme multilayers, were fabricated via the layer-by-layer self-assembly method. Glucose oxidase (GOD), horseradish peroxidase (POD), or preformed enzymepolyelectrolyte complexes were assembled in alternation with oppositely charged polyelectrolytes onto PS particles. Microelectrophoresis, single-particle light scattering, and transmission electron microscopy confirmed stepwise growth of the multilayer films on the colloid particles. The high surface area enzyme multilayer-coated particles were successfully employed as specific enzyme reactors (i.e., as catalysts). Whereas no loss in activity was observed for the enzymes immobilized directly onto particle surfaces, precomplexing the enzymes with polymer in solution drastically reduced their activity (by up to 70%). The enzymatic activity (per particle) was found to increase with the number of enzyme layers immobilized, irrespective of whether the enzyme was precomplexed. Ho...

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