Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.
摘要：
The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.
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关键词：
Biotin Oligodeoxyribonucleotides Oligoribonucleotides Fluorescent Dyes Nucleic Acid Hybridization Isotope Labeling
DOI：
10.1093/nar/14.15.6227
被引量：
年份：
1986
Taylor & Francis
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