fusion protein
申请(专利)号：
US201515533378
申请日期：
2015-12-08
公开/公告号：
US2017362279A1
公开/公告日期：
2017-12-21
申请(专利权)人：
LIFE SCIENCE INKUBATOR BETRIEBS GMBH & CO. KG
国省代号：
DE
被引量：
摘要：
Liposome preparation. Liposomes were prepared following the method of Hope et al. (1985). Briefly, dried lipid films were hydrated with 5 mM HEPES, 150 mM NaCl, 0?1 mM EDTA (pH 7?4) and sub- jected to five cycles of freezing and thawing. Subsequently, vesicles were sized by extrusion through a polycarbonate filter with a pore size of 0?1 mm. Liposomes consisted of a 1 : 1 : 1 : 1?5 molar ratio of phos- phatidylcholine (from egg yolk), phosphatidylethanolamine (prepared from egg phosphatidylcholine by transphosphatidylation), sphingo- myelin (from bovine brain) and cholesterol. All lipids were purchased from Avanti Polar Lipids. The concentration of liposome suspensions was determined by phosphate analysis (Bo¨ttcher et al., 1961).
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