The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator

阅读量:

411

作者:

DR MccartyT HattoriCB CarsonV VasilM LazarIK Vasil

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摘要:

The Viviparous-1 (Vp1) gene of maize is specifically required for expression ofthe maturation program in seed development. We show that Vp1 encodes a 73,335dalton protein with no detectable homology to known proteins. An acidictranscriptional activation sequence was identified by fusion to the GAL4 DNA-binding domain. Expression of VP1 in maize protoplasts resulted in strongactivation (greater than 130-fold) of a reporter gene fused to the promoter of apresumptive target gene. The acidic domain in VP1 was essential fortransactivation and could be functionally replaced by the activator sequence ofthe herpes simplex virus VP16 protein. Our results indicate that VP1 is a noveltranscription factor possibly involved in potentiation of a seed-specifichormone response.

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DOI:

10.1016/0092-8674(91)90436-3

被引量:

1457

年份:

1991

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来源期刊

引用走势

1998
被引量:102

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