FACT Facilitates Transcription-Dependent Nucleosome Alteration
摘要：
The FACT (facilitates chromatin transcription) complex is required for transcript elongation through nucleosomes by RNA polymerase II (Pol II) in vitro. Here, we show that FACT facilitates Pol II-driven transcription by destabilizing nu-cleosomal structure so that one histone H2A-H2B dimer is removed during enzyme passage. We also demonstrate that FACT possesses intrinsic histone chaperone activity and can deposit core histones onto DNA. Importantly, FACT activity requires both of its constituent subunits and is dependent on the highly acidic C terminus of its larger subunit, Spt16. These findings define the mechanism by which Pol II can transcribe through chromatin without disrupting its epigenetic status.
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关键词：
Rna-polymerase-ii Saccharomyces-cerevisiae In-vivo Elongation Chromatin Protein Complex Pob3 Ssrp1 Cdc68
DOI：
10.1126/science.1085703
被引量：
年份：
2003
Taylor & Francis
掌桥科研
万方医学
Semantic Scholar
dx.doi.org
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