Neuroepithelial Stem Cells from the Embryonic Spinal Cord: Isolation, Characterization, and Clonal Analysis - ScienceDirect

来自 dx.doi.org

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阅读量：

113

作者：

A Kalyani，K Hobson，MS Rao

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摘要：

Adherent cultures of E10.5 neuroepithelial cells (cells) from the caudal neural tube require (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. () does not support E10.5 NEP cells in adherent culture and cells do not form -dependent neurospheres. cells, however, can be grown as -dependent neurospheres. cells express and lack all lineage-specific markers for neuronal and glial sublineages, retain their pleuripotent character over multiple passages, and can differentiate into neurons, astrocytes, and oligodendrocytes when plated on laminin in the absence of CEE. In clonal culture, cells undergo self-renewal and generate colonies that vary in size from single cells to several thousand cells. With the exception of a few single-cell clones, all other -derived clones contain more than one identified phenotype, with over 40% of the colonies containing A2B5, beta-111 , and -immunoreactive cells. Thus, cells are multipotent and capable of generating multiple neural derivatives. cells also differentiate into motoneurons immunoreactive for transferase () and the low-affinity neurotrophin receptor () in both mass and clonal culture. Double labeling of clones for and glial, neuronal, or oligodendrocytic lineage markers shows that motoneurons always arose in mixed cultures with other differentiated cells. Thus, cells represent a common progenitor for motoneurons and other spinal cord cells. The relationship of cells with other neural stem cells is discussed.

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关键词：

proline hippocampus glutamate uptake development

DOI：

10.1006/dbio.1997.8592

被引量：

690

年份：

1997