Powerful and versatile enhancer-promoter unit for mammalian expression vectors
摘要：
The enhancer and promoter of the immediate early gene of human cytomegalovirus (hCMV) were tested as a transcriptional control element by fusion to the cat gene, followed by measurement of its expression from plasmid DNA in the extrachromosomal and integrated state, respectively. Comparison of the hCMV enhancerpromoter to two other viral elements was performed in six commonly used cell lines of different tissue and species origin. Irrespective of the cell line and of the state of the DNA, the hCMV enhancer-promoter was considerably stronger than both the SV40 promoter and the long terminal repeat of Rous sarcoma virus.
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关键词：
Ap ampicillin ampicillin bp base pair(s base pair(s CAT Cm acetyltransferase Cm acetyltransferase cat gene coding for CAT gene coding for CAT Cm chloramphenicol chloramphenicol
DOI：
10.1016/0378-1119(86)90137-X
被引量：
年份：
1986
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