Tyrosine kinases activate store-mediated Ca2+ entry in human platelets through the reorganization of the actin cytoskeleton

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摘要：

We have recently reported that store-mediated Ca2+ entry in platelets is likely to be mediated by a reversible trafficking and coupling of the endoplasmic reticulum with the plasma membrane, a model termed 'secretion-like coupling'. In this model the actin cytoskeleton plays a key regulatory role. Since tyrosine kinases have been shown to be important for Ca2+ entry in platelets and other cells, we have now investigated the possible involvement of tyrosine kinases in the secretion-like-coupling model. Treatment of platelets with thrombin or thapsigargin induced actin polymerization by a calcium-independent pathway. Methyl 2, 5-dihydroxycinnamate, a tyrosine kinase inhibitor, prevented thrombin- or thapsigargin-induced actin polymerization. The effects of tyrosine kinases in store-mediated Ca2+ entry were found to be entirely dependent on the actin cytoskeleton. PP1, an inhibitor of the Src family of proteins, partially inhibited store-mediated Ca2+ entry. In addition, depletion of intracellular Ca2+ stores stimulated cytoskeletal association of the cytoplasmic tyrosine kinase pp60(arc), a process that was sensitive to treatment with cytochalasin D and PPI, but not to inhibition of Ras proteins using prenylcysteine analogues. Finally, combined inhibition of both Ras proteins and tyrosine kinases resulted in complete inhibition of Ca2+ entry, suggesting that these two families of proteins have independent effects in the activation of store-mediated Ca2+ entry in human platelets. [References: 44]

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关键词：

PG_Thesis

DOI：

10.1007/s00253-004-1774-4

被引量：

161

年份：

2000