摘要：

For a classical biochemist, it was difficult to visualize enzymes catalyzing reactions in the absence of water, i.e. in non-aqueous media. Addition of organic solvents was done either to precipitate enzymes or to study denaturation. Thus, the study of enzyme action in organic solvents is a comparatively recent aspect of enzymology. Singer (1962) reviewed the status of this area and referred to it as a 'rapidly developing area of biophysical chemistry'. While we have made only limited advances since then in our understanding of biophysical chemistry of non-aqueous enzymology, its biotechnological implications have resulted in an explosive growth of the literature (Linhardt, 1986; Dordick, 1988).

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