Glyoxylate Regeneration Pathway in the Methylotroph Methylobacterium extorquens AM1
摘要：
Most serine cycle methylotrophic bacteria lack isocitrate lyase and convert acetyl coenzyme A (acetyl-CoA) to glyoxylate via a novel pathway thought to involve butyryl-CoA and propionyl-CoA as intermediates. In this study we have used a genome analysis approach followed by mutation to test a number of genes for involvement in this novel pathway. We show that methylmalonyl-CoA mutase, an R-specific crotonase, isobutyryl-CoA dehydrogenase, and a GTPase are involved in glyoxylate regeneration. We also monitored the fate of (14)C-labeled carbon originating from acetate, butyrate, or bicarbonate in mutants defective in glyoxylate regeneration and identified new potential intermediates in the pathway: ethylmalonyl-CoA, methylsuccinyl-CoA, isobutyryl-CoA, methacrylyl-CoA, and beta-hydroxyisobutyryl-CoA. A new scheme for the pathway is proposed based on these data.
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关键词：
Methylobacterium extorquens Glyoxylates Coenzyme A GTP Phosphohydrolases Enoyl-CoA Hydratase Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors Methylmalonyl-CoA Mutase DNA, Bacterial Base Sequence
DOI：
10.1128/JB.184.6.1750-1758.2002
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年份：
2002
Semantic Scholar
万方医学
掌桥科研
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