Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity
摘要：
Background Nanomaterials such as SiO2 nanoparticles (SiO2NP) are finding increasing applications in the biomedical and biotechnological fields such as disease diagnostics, imaging, drug delivery, food, cosmetics and biosensors development. Thus, a mechanistic and systematic evaluation of the potential biological and toxic effects of SiO2NP becomes crucial in order to assess their complete safe applicability limits. Results In this study, human monocytic leukemia cell line THP-1 and human alveolar epithelial cell line A549 were exposed to a range of amorphous SiO2NP of various sizes and concentrations (0.01, 0.1 and 0.5 mg/ml). Key biological indicators of cellular functions including cell population density, cellular morphology, membrane permeability, lysosomal mass/pH and activation of transcription factor-2 (ATF-2) were evaluated utilizing quantitative high content screening (HCS) approach and biochemical techniques. Despite the use of extremely high nanoparticle concentrations, our findings showed a low degree of cytotoxicity within the panel of SiO2NP investigated. However, at these concentrations, we observed the onset of stress-related cellular response induced by SiO2NP. Interestingly, cells exposed to alumina-coated SiO2NP showed low level, and in some cases complete absence, of stress response and this was consistent up to the highest dose of 0.5 mg/ml. Conclusions The present study demonstrates and highlights the importance of subtle biological changes downstream of primary membrane and endocytosis-associated phenomena resulting from high dose SiO2NP exposure. Increased activation of transcription factors, such as ATF-2, was quantitatively assessed as a function of i) human cell line specific stress-response, ii) SiO2NP size and iii) concentration. Despite the low level of cytotoxicity detected for the amorphous SiO2NP investigated, these findings prompt an in-depth focus for future SiO2NP-cell/tissue investigations based on the combined analysis of more subtle signalling pathways associated with accumulation mechanisms, which is essential for establishing the bio-safety of existing and new nanomaterials.
展开
关键词：
DOI：
10.1186/1477-3155-9-29
被引量：
年份：
2011
Taylor & Francis
Springer
(全网免费下载)
Springer
Wiley
Semantic Scholar
(全网免费下载)
查看更多
掌桥科研
dx.doi.org
BioMed Central
(全网免费下载)
NCBI
BioMed Central
(全网免费下载)
Europe PMC
(全网免费下载)
NCBI
BioMed Central
Europe PMC
OALib
ResearchGate
ResearchGate
(全网免费下载)
EBSCO
Springer Nature
(全网免费下载)
biomedsearch.com
medicine.tcd.ie
(全网免费下载)
medicine.tcd.ie
(全网免费下载)
lenus.ie
(全网免费下载)
aac.asm.org
tara.tcd.ie
(全网免费下载)
edepositireland.ie
(全网免费下载)
core.kmi.open.ac.uk
jnanobiotechnology.com
(全网免费下载)
edepositireland.ie
pubfacts.com
jnanobiotechnology.com
lenus.ie
(全网免费下载)
学术范
(全网免费下载)
go.galegroup.com
core.ac.uk
mailer.oalib.net
readcube.com
tcd.ie
(全网免费下载)
tara.tcd.ie
(全网免费下载)
tara.tcd.ie
science-open.com
hdl.handle.net
rug.nl
springerprofessional.de
学术范
d.scholar.cnki.net
core.ac.uk
(全网免费下载)
lenus.ie
rian.ie
scienceopen.com
biomedcentral.com
(全网免费下载)
Springer
(全网免费下载)
Semantic Scholar
(全网免费下载)
Europe PMC
(全网免费下载)
BioMed Central
(全网免费下载)
BioMed Central
(全网免费下载)
ResearchGate
(全网免费下载)
tara.tcd.ie
(全网免费下载)
edepositireland.ie
(全网免费下载)
lenus.ie
(全网免费下载)
lenus.ie
(全网免费下载)
medicine.tcd.ie
(全网免费下载)
tara.tcd.ie
(全网免费下载)
tcd.ie
(全网免费下载)
biomedcentral.com
(全网免费下载)
jnanobiotechnology.com
(全网免费下载)
core.ac.uk
(全网免费下载)
medicine.tcd.ie
(全网免费下载)
Springer Nature
(全网免费下载)
学术范
(全网免费下载)
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
