Regulation of Hsp27 Oligomerization, Chaperone Function, and Protective Activity against Oxidative Stress/Tumor Necrosis Factor ␣ by Phosphorylation*
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Quentin, Fallavier, France) was analyzed. After 24 h of incubation in the presence of 10 M menadione, cells were harvested by trypsination, resuspended in phosphate-buffered saline, and incubated with 1 mg/ml propidium iodide (PI). After 10 min of incubation at room temperature, cell fluorescence was recorded by flow cytometric analysis using a FACScan cytometer (Beckton Dickinson, Le Pont de Claix, France) equipped with FL1 and FL3 detectors. Excitation: 488 nm, emission filters 530 nm for GFP and 630 nm for PI.
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