摘要：

(57) [summary] The present invention relates to a method for enzymatically synthesizing pure oligonucleotide chirally. In particular, using digestible template optionally is capable of directing the synthesis of oligonucleotide products that can be cut arbitrarily into individual oligonucleotides of two or more optionally present invention, 3'-ribonucleotides relates to a method of using a primer, it is enzymatically synthesized oligonucleotides. In this way, it can be synthesized at the same time two or more oligonucleotides having a sequence length and / or the same or different. Wherein the restriction endonuclease recognition site given that are identified as capable of cleaving phosphorothioate oligonucleotide in the present invention and containing at least one phosphorothioate linkage, optionally, therefore, oligonucleotides, and the separation of synthetic oligonucleotides after purification is facilitated.

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