摘要：

The concentrations of L-3-hydroxybutyrate (L-3HB) in rat various tissues were determined by column-switching HPLC with pre-column fluorescence derivatization. The tissues of 4, 8, 16, 24-week-old male Sprague–Dawley rats were homogenized and the supernatants were derivatized with a fluorescent reagent, 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ), then separated on an ODS column followed by two-OD-RH-connected chiral columns for enantioseparation. The results showed that the contents of L-3HB were highest in the heart (2.10 ± 1.10 μM, 8 weeks of age). In the adrenal gland, the percentage of L-3HB was found to compose as high as 43.4% of total 3HB, followed by 28.6% in heart homogenate. We further investigated L-3HB levels in heart-derived H9c2 cell line in the presence or absence of 0-10 μM (D+L)-3HB, L-3HB, D-3HB in the medium. After incubation with 2 mM D-3HB for 10 hrs, the concentration of L-3HB in the cells was increased significantly. The result suggests that D-3HB is a precursor of L-3HB.

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