摘要：

FIG. 2. Efficiency of intranuclear (A) and cytoplasmic (B) cDNA microinjections in COS-7 cells. In A and B (left panels) dose-effect relations with naked pCMV-LacZ cDNA (open symbols) or with PEI⅐cDNA complexes at 5 equivalents (filled symbols) are shown. A variable number of injected plasmid copies as indicated on the x axis was used. Data are mean Ϯ S.E., with n between 178 and 650. Right panels show the effects of varying the PEI/cDNA ratio on injection efficiency. Cells were injected with 10,000 plasmid copies. Stars indi- cate significant difference, with p Ͻ 0.05 (*) or p Ͻ 0.001 (***) between cells injected with naked DNA and cells injected with DNA complexed with PEI.

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