Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics

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160

作者：

MA Grandbastien，A Spielmann，M Caboche

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摘要：

Transposable elements can be identified by their ability to induce mutant alleles at new loci. The retrotransposon family is thought to transpose through an RNA intermediate and has many similarities to vertebrate proretroviruses. In plants, retrotransposons have been described in maize,and wheat, and non-viral retroposons in maize. Most of these elements, however, have been found as non-mobile integrated units. Here, we report the isolation of the first tobacco () transposable element, Tnt1, which seems to be the most complete mobile retrotransposon characterized in higher plants. Tntl has been isolated after its transposition into the nitrate reductase (NR) structural gene of tobacco, and transposition events have been detected throughselection of spontaneous NR-deficient (NR) mutant lines in cell cultures derived from tobacco mesophyll protoplasts. Tntl is 5,334 nucleotides long, contains two 610-base-pair-long terminal repeats and a single open reading frame of 3,984 nucleotides. Comparison of the Tntl open reading frame coding potential with those of theretrotransposon, yeast Ty retrotransposon, and vertebrate proretrovirusesrevealed that Tntl is closely related toand carries all the functions known to be required for autonomous transposition by reverse transcription.

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关键词：

DNA Transposable Elements Restriction Mapping Crosses, Genetic Base Sequence Mutation Amino Acid Sequence Molecular Sequence Data Alleles Plants Plants, Toxic