Metabolism of (-)-(S)-nicotine in the isolated perfused rabbit lung.

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作者：

G Aislaitner，A Bello，SC Tan，AJ Hutt，JW Gorrod

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摘要：

The metabolism of (−)-( S )-nicotine has been investigated following intratracheal administration to the recirculating perfused rabbit lung model. The metabolic products present in the perfusate were identified by co-chromatography (HPLC and GC) with authentic standards and quantified by HPLC. After the 180 min perfusion period, nicotine was found to be metabolically transformed to cotinine (33.7%), 3-hydroxycotinine (10.4%), cotinine-1-N-oxide (3.4%) and nicotine-1′-N-oxide (14.4%). Norcotinine, nornicotine, 3-pyridyl-4-oxo-N-methylbutyramide and an uncharacterised metabolite were also detected in low amounts. Following the perfusion experiment, part of the lung tissue was homogenised in the presence of [ 14 C]-sodium cyanide. Subsequent analysis of the homogenates indicated the formation of 2′-cyanonicotine, 1′-cyanomethylnornicotine and the diastereoisomeric 5′-cyanonicotines.

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关键词：

Nicotine metabolism isolated perfused lung model 14C]-cyanide trapping technique radiolabelled cyanonicotines

DOI：

10.1007/BF03190976

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年份：

1997