A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium
摘要：
A rapid technique is described for the isolation of muscle cells from adult rat myocardium using in vitro perfusion with calcium-free bicarbonate buffer containing crude collagenase. Under optimum conditions, 5 × 10 6 cells per g tissue are obtained and the suspension may be purified to contain 70% intact cells. The complete procedure is rapid and isolated myocytes beat, exclude vital stains, have conventional sub-cellular morphology, show tight respiratory coupling and also have a tolerance to external calcium not found in cells isolated by other perfusion techniques. This represents a significant advance in the development of an isolated cell model for the study of myocardial mechanisms.
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DOI：
10.1016/0006-291X(76)90997-9
被引量：
年份：
1976
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