摘要：

Objective:Using phage display technique to display human CD28,CD137 on phage and detect its anti- genicity and bioactivity.Methods:Construct recombinant phagemid vector pComb3H-CD28 and pComb3H- CD137.Display CD28 and CD137 on phage surface.The antigenicity and bioactivity of CD28 and CD137 displayed on phage was detected by ELISA,FCM and inhibition assay of lymphocyte proliferation(MTT).Results:The recom- binant phagemid vector pComb3H-CD28 and pComb3H-CD137 was successfully constructed.CD28 and CD137 was displayed on phage.ELISA detection showed that phage-displayed CD28 could bind to CD28Ab and CD80; FCM detection indicated that phage-displayed CD28 could competitively bind to CD28Ab with CD28 on the sur- face of Jurkat cell and could also bind to Raji cell surface CD80,these data demonstrated that phage-displayed CD28 not only possesses antigenicity but also possesses its native structure binding to its ligand.ELISA detection showed that phage-displayed CD137 could bind to CD137Ab,it has CD137 antigenicity.In further,MTT inhibition assay showed that lymphocytes proliferation stimulated by CD28Ab,CD137Ab could be inhibited by phage-dis- played CD28,CD137 respectively.Conclusion:The studies demonstrate that phage display system can display hu- man CD28 extra-cellular domain homodimer with antigenicity and bioactivity.

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