摘要：

Purpose:To establish an effective TLC method for the analysis of several monosaccharides and oligosaccharides and determinate the content of stachyose in in oligosaccharide from Stachys seiboibi Miq.Methods:TLC with different developing systems was applied to the analysis of fructose,sucrose,raffinose and stachyose,related to the degree of isolated spots,the spots focusing on it or not,tailing phenomenon and expanding time.TLCS was applied to determinate the content of stachyose in oligosaccharide from Stachys seiboibi Miq.Results:A mixed solution composed of ethyl acetate,methanol,water and glacial acetic acid in a ratio of ethyl acetate:methanol:water:glacial acetic acid=11.5:3.2:2:3.2 by volume was used as development reagent.A solution containing anisaldehyde and concentrated sulfuric acid was used as color reagent.The content of stachyose in oligosaccharide from Stachys seiboibi Miq was 69.3%.

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