Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase
摘要：
p21-activated kinases (PAKs) are implicated in the cytoskeletal changes induced by the Rho family of guanosine triphosphatases. Cytoskeletal dynamics are primarily modulated by interactions of actin and myosin II that are regulated by myosin light chain kinase (MLCK)–mediated phosphorylation of the regulatory myosin light chain (MLC). p21-activated kinase 1 (PAK1) phosphorylates MLCK, resulting in decreased MLCK activity. MLCK activity and MLC phosphorylation were decreased, and cell spreading was inhibited in baby hamster kidney–21 and HeLa cells expressing constitutively active PAK1. These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation.
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关键词：
Cell Line Hela Cells Cytoskeleton Animals Humans Diacetyl Myosins GTP Phosphohydrolases GTP-Binding Proteins cdc42 GTP-Binding Protein
DOI：
10.1126/science.283.5410.2083
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年份：
1999
Wiley
万方医学
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