Multiple beta 1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells.

阅读量：

作者：

展开

摘要：

Mammalian cell receptors that promote entry of intracellular bacteria into nonphagocytic cells have not been identified. We show here that multiple members of the integrin superfamily of cell adhesion receptors bind the Y. pseudotuberculosis invasin protein prior to bacterial penetration into mammalian cells. Affinity chromatography of crude detergent extracts demonstrated that integrins containing the subunit structures α 3 β 1, α 4 β 1, α 5 β 1, and α 6 β 1 bound to immobilized invasin. Furthermore, phospholipid vesicles containing isolated integrin proteins were able to attach to invasin. Specificity for invasin binding to the identified integrin receptors was also demonstrated, as immunoprobing and phospholipid reconstitution studies showed that the α 2 β 1 integrin, β 2 chain integrins, and vitronectin receptor ( α v β 3) were not involved in cellular attachment to invasin.

展开

关键词：

PHOTOSYNTHESIS SPINACH CHLOROPLASTS AMPHIPHILIC AMINES ESR SPECTROSCOPY MEMBRANE PERTURBATION

DOI：

10.1016/0092-8674(90)90099-Z

被引量：

1213

年份：

1990