Functional expression of constitutive nitric oxide synthases regulated by voltage-gated Na+ and Ca2+ channels in cultured human astrocytes (p 53-62)
摘要：
Wereportthefunctionalcharacterizationofconstitutivenitricoxidesynthase(s)(NOS)suchasneuronalandendothelialNOSinculturedhumanastrocytesExposureofculturedhumanastrocytesto1μMveratridineor50mMKClproducedapronouncedincreaseinacalmodulin-dependentNOSactivityestimatedfromcGMPformationThefunctionalexpressionofvoltage-gatedNa+channel,whichisestimatedbytheresponsetoveratridine,appearedtobeearlier(atseconddayinculture)thanthatofvoltage-gatedCa2+channels,whichareestimatedbytheresponsetotheKClstimulation(atfourthdayinculture)TheKCl-evokedNOsynthesiswastotallyreversedbyL-typeCa2+channelblockerssuchasnifedipineandverapamil,butnotbyω-conotoxinGVIA,anN-typeCa2+channelblocker,orω-agatoxinIVA,aP/Q-typeCa2+channelblockerInaddition,verapamilabolishedtheKCl-inducedincreaseintheintracellularfreeCa<s
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关键词：
cultured human astrocytes nitric oxide synthase voltage-gated Ca2+ channel voltage-gated Na+ channel cGMP
DOI：
10.1002/glia.10359
被引量：
年份：
2010
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