METHODS AND PRODUCTS FOR THE SYNTHESIS OF OLIGOSACCHARIDE STRUCTURES ON GLYCOPROTEINS, GLYCOLIPIDS, OR AS FREE MOLECULES
申请(专利)号:
EP19910905027
申请日期:
1991-02-14
公开/公告号:
EP0515536B1
公开/公告日期:
2000-01-19
申请(专利权)人:
UNIV MICHIGAN
摘要:
the gene product obtained by expression of the gene, is obtained by a process consisting of (i) to isolate a first cell having a characteristic of post - translation of interest, the characteristic of post translation is the presence of an oligosaccharide, or a polysaccharide of interest linked to a membrane on the surface of the first cell.the presence of an oligosaccharide or polysaccharide extract of soluble in the first cell.the presence of a specific glycosyltransferase activity in an extract of the first cell; and (ii) to create a library of cdna or genomic dna as the genetic material of the first single cell; (iii) transforming cell o using the library hosts; and (iv) to sort out those host cells transformed in order to obtain a cellwith the feature of domestic guest post translationally, producing a cell containing the gene.
展开
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用!