METHODS AND PRODUCTS FOR THE SYNTHESIS OF OLIGOSACCHARIDE STRUCTURES ON GLYCOPROTEINS, GLYCOLIPIDS, OR AS FREE MOLECULES

阅读量:

5

申请(专利)号:

EP19910905027

申请日期:

1991-02-14

公开/公告号:

EP0515536B1

公开/公告日期:

2000-01-19

申请(专利权)人:

UNIV MICHIGAN

发明人:

JB Lowe

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摘要:

the gene product obtained by expression of the gene, is obtained by a process consisting of (i) to isolate a first cell having a characteristic of post - translation of interest, the characteristic of post translation is the presence of an oligosaccharide, or a polysaccharide of interest linked to a membrane on the surface of the first cell.the presence of an oligosaccharide or polysaccharide extract of soluble in the first cell.the presence of a specific glycosyltransferase activity in an extract of the first cell; and (ii) to create a library of cdna or genomic dna as the genetic material of the first single cell; (iii) transforming cell o using the library hosts; and (iv) to sort out those host cells transformed in order to obtain a cellwith the feature of domestic guest post translationally, producing a cell containing the gene.

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