摘要：

[Objectives]Pseudomonas taiwanensis LH-3 was immobilized successfully lo degrade lawsone and die possible underlying mechanism was anticipated to be elucidated in this study. [MethodsjThe strain was immobilized in sodium alginate beads in which biochar(the masson pine tree trunk were pyrolyzed at 700 °C )was added lo enhance the removal efficiency. The morphological analysis of the joint immobilized beads was conducted. The degradation behavior of the immobilized bacteria was tentatively investigated along with control assays in terms of different environmental conditions. In addition, the reuse ability was comparatively investigated. Finally, the operation performance of sequencing batch reactor ( SBR) with immobilized strain was systematically evaluated. [Results] Brunner-Eimnet-Teller( BET) results showed that the specific surface area of joint immobilization beads and SA bacteria beads were 22.26 and 4.02 m2·g-1, respectively. The adsorption-degradation performance of joint immobilization strains favored the degradation process. The joint immobilized bacteria showed better abilities than free bacteria in adapting to a wide range of temperature, pll, initial concentration of lawsone and heavy metal. Moreover, joint immobilized strains can be successfully reused for more than 9 times. In laboratory-scale SBR, the joint immobilized strain can degrade lawsone in a stable way. [Conclusions] Biochar can increase the surface area of sodium alginate beads and enhance the overall degradation efficiency of immobilized strain. Finally, the jointed immobilized beads can adopt to boarder environmental conditions and possess better application capability.

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