Microbial pathogenesis in cystic fibrosis: co-ordinate regulation of heat-shock response and conversion to mucoidy in Pseudomonas aeruginosa.
摘要:
Conversion of Pseudomonas aeruginosa to the mucoid phenotype plays a major role in the pathogenesis of respiratory infections in cystic fibrosis (CF). One mechanism responsible for mucoidy is based on mutations that inactivate the anti-σ factor, MucA, which normally inhibits the alternative sigma factor, AlgU. The loss of MucA allows AlgU to freely direct transcription of the genes responsible for the production of the exopolysaccharide alginate resulting in mucoid colony morphology. In Escherichia coli , a close homologue of AlgU, σ E , directs transcription of several genes under conditions of extreme heat shock. Here we examined whether AlgU, besides its role in controlling alginate production, affects the heat-shock response in P. aeruginosa . The P. aeruginosa rpoH gene encoding a homologue of the major heat-shock sigma factor, σ 32 , was found to be transcribed by AlgU containing RNA polymerase from one of its promoters (P 3 ) identified in this study. Transcription of rpoH from P 3 was elevated upon exposure to extreme heat shock in an algU -dependent manner. Importantly, the AlgU-dependent promoter of rpoH was found to be activated in mucoid mucA mutants. In keeping with this observation, introduction of a wild-type mucA gene abrogated AlgU-dependent rpoH transcription in mucoid P. aeruginosa laboratory isolates and CF isolates. These results suggest that conversion to mucoidy and the heat-shock response are co-ordinately regulated in P. aeruginosa . The simultaneous activation of both systems in mucA mutants, selected in the lungs of CF patients, may have significance for the inflammatory processes characteristic of the establishment of chronic infection and ensuing clinical deterioration in CF.
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关键词:
Alternate sigma-factor Escherichia-coli Rna-polymerase Brucella-abortus High-temperature Gene-expression Stress-response Alginate production Rpoh gene Cloning
DOI:
10.1046/j.1365-2958.1997.3411711.x
被引量:
年份:
2010

























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