Nucleotide sequence of the 3' terminal third of rabbit alpha-globin messenger RNA: comparison with human alpha-globin messenger RNA.

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作者：

NJ Proudfoot，S Gillam，M Smith，JI Longley

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摘要：

Rabbit and human α-globin complementary DNA was synthesized using the primer (dT 10)dGdCdC hybridized to globin mRNA with reverse transcriptase, and sequenced using the plus and minus gel sequencing procedure ( Sanger and Coulson, 1975; Brownlee and Cartwright, 1977). The complete 3′ noncoding region, 89 nucleotides in length, and one third of the coding region of rabbit α-globin mRNA have thus been sequenced. These data are compared with the near complete 3′ noncoding region sequence of human α-globin mRNA obtained in these and previous studies. The two mRNAs are approximately 80% homologous in their 3′ noncoding regions, except that the human sequence has an insert of 24 nucleotides. A very similar insert in sequence has been described in human β-globin mRNA ( Proudfoot, 1977).

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关键词：

Animals Rabbits Humans Deoxyribonucleases Oligonucleotides DNA Globins RNA-Directed DNA Polymerase Nucleotides Base Sequence