摘要：

Improvingcell-material interactions of nonadhesive scaffolds iscrucial for the success of biomaterials in tissue engineering. Dueto their high surface area and open pore structure, sponges are widelyreported as absorbent materials for biomedical engineering. The biocompatibilityand biodegradability of polysaccharide sponges, coupled with the chemicalfunctionalities of supramolecular dimers, make them promising combinationsfor the development of adhesive scaffolds. Here, a supramoleculartactic based on (UPy)-modified polysaccharide associated with three-dimensionalstructure of sponges was developed to reach enhanced cellular adhesion.For this purpose, three approaches were examined individually in orderto accomplish this goal. In the first approach, the backbone polysaccharideswith noncell adhesive properties were modified via a modular tacticusing UPy-dimers. Hereupon, the physical–chemical characterizationsof the supramolecular sponges were performed, showing that the presenceof supramolecular dimers improved their mechanical properties andinduced different architectures. In addition, small-angle neutronscattering (SANS) measurements and rheology experiments revealed thatthe UPy-dimers into agarose backbone are able to reorganize in thinningaggregates. It is also demonstrated that the resulted UPy-agarose(AGA-UPy) motifs in surfaces can promote cell adhesion. Finally, thelast approach showed the great potential for use of this novel materialin bioadhesive scaffolds indicating that neural stem cells show aspreading bias in soft materials and that cell adhesion was enhancedfor all UPy-modified sponges compared to the reference, i.e. unmodifiedsponges. Therefore, by functionalizing sponge surfaces with UPy-dimers,an adhesive supramolecular scaffold is built which opens the opportunityits use neural tissues regeneration.

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