摘要：

Objective:To investigate the immunomodulation of CCK8 on the Coxsackievirus B(CVB)-attacked human peripheral blood plasmacytoid dendritic cells(p DC).Methods:Peripheral blood mononuclear cells of healthy volunteers were separated by Ficoll-Hypaque gradient density centrifugation.The p DC was separated and divided into five groups,which were the control group,CVB attacked group,the group of CCK8 treated after CVB attack,the group of PGE2 treated after CVB attack and the group of CCK8+PGE2 treated after CVB attack.100-time TCID50 of CVB was applied for the attack on p DC.Real-time PCR and Immunofluorescence technique were employed to detect the expression of CCK1 R/CCK2 R mRNA and protein.Then,the expression levels of costimulatory molecules such as CD80,CD86,HLA-DR ligand,and the chemokine receptor CCR7 were evaluated by Flow Cytometry Analysis.The supernatants of p DCs were collected,and the content of IFN-α was determined by Enzyme-linked Immunosorbent Assay.Results:CCK1 R and CCK2 R were co-expressed in human peripheral blood p DC,and both were significantly upregulated after CVB attack in vitro.Expression of CD80,CD86,HLA-DR and IFN-α were decreased in the CVB + CCK8 group compared with the CVB group,which suggested that CCK8 may reduce the CVB activation of p DC.Whereas expression of CD80,CD86,HLA-DR,CCR7 and IFN-α were increased in the CVB + PGE2 group compared with the CVB group,which suggested that PGE2 may increase the CVB activation of p DC in vitro.Conclusion:CCK8 repressed the CVB-attacked p DC,while PGE2 activated the CVB-attacked p DC.

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