摘要：

Objective:To investigate the antipruritic effect and antipruritic mechanism of Xiaofeng Powder in AEW animal itching model. Methods:Male C57/BL6 mice were randomly divided into AEW models(modeling method:after shaving on the back,acetone:ether = 1:1 wet compress for 15 seconds,wet water for 30 seconds,morning and evening 2 times,for 7 days) According to the saline group,the AEW model Xiaofeng Powder low-dose group,and the AEW model Xiaofeng Powder high-dose group,the behavioral video recording method was used to evaluate the degree of itchiness of the mouse hind paws scratching the back modeling area;HE staining observation Morphological changes of skin lesions;correlation analysis of skin and DRG tissues in mouse model area by fluorescence real-time quantitative PCR,and observation of changes in mRNA expression of TRPA1,TRPV1 and TRPV4,and evaluation of itching Efficacy;Western blotting was used to observe the phosphorylation levels of the above three itch-related proteins,and the antipruritic effect of Xiaofeng Powder was evaluated. Results:The preventive administration of Xiaofeng Powder low-dose and high-dose groups reduced the scratching behavior of AEW model mice. The low-dose group had significant difference from the 4 th day to the 7 th day of the model(P0.05). The high-dose group showed significant difference from the third day to the seventh day of the model(P0.01),and it was dose-dependent. The anti-itch effect of the high-dose group was better than that of the low-dose group;the low-dose,high-dose group The thickness of the epidermis and the tight junction of the skin tissue were all lighter than that of the control saline group. The results of Q-RT-PCR showed that the mRNA expression level of TRPA1/V1/V4 was up-regulated in the saline group after modeling,and the wind was low. The high-dose group was down-regulated,and there was a significant difference(P0.05). The WB results showed that the phosphorylation level of TRPA1/V1/V4 was up-regulated in the saline group after model establishment,while the low-dose,high-dose group was down-regulated. There was a significant difference(P0.05). Conclusion:Xiaofeng Powder has an inhibitory effect on pruritus caused by AEW model in a dose-dependent manner. The mechanism of Xiaofeng Powder inhibiting pruritus in AEW model may be related to the mediation of TRPA1,TRPV1 and TRPV4.

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