A novel graphene oxide/chitosan foam incorporated with metal–organic framework stationary phase for simultaneous enrichment of glycopeptide and phosphopeptide with high efficiency
摘要:
A novel hydrophilic porous biocomposite was fabricated by incorporating graphene oxide (GO) @chitosan (CS) foam substrate (GO@CS@ZIF-8 foam) with ZIF-8 crystals in situ via a facile stirring method for simultaneous enrichment of glycopeptides and phosphopeptides from complex biological samples. The experimental results demonstrated that GO@CS@ZIF-8 foam exhibited favorable specificity for simultaneous enrichment of N-glycopeptides and phosphopeptides under the same condition for HRP and β-casein tryptic digest mixtures. The novel material was further applied to enriching both glycopeptides and phosphopeptides simultaneously from 4 μL complex human serum, and 423N-glycopeptides and 40 phosphopeptides corresponding to 133 glycoproteins and 29 phosphoproteins were identified, respectively.
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关键词:
Mass spectrometry Glycopeptides enrichment Phosphopeptides enrichment Hydrophilic porous biocomposite Foam MASS-SPECTROMETRY SELECTIVE ENRICHMENT OXIDE NANOCOMPOSITE POLYURETHANE FOAM EXTRACTION SHELL BIOMARKERS STRATEGY MOFS
DOI:
10.1007/s00216-021-03861-z
年份:
2022
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