箬叶中抑菌成分的提取及抑菌试验
摘要:
Us disc diffusion method, and investigate the antimicrobial actives of the extracts from Aspidistra elatior Blume, with Staphylococcus aureus and Escherichia coli as indicative bacteria .The extraction technology was opti-mized with disc diffusion method.With the response surface methodology test , the results showed that the optimum extracting condition was:extraction temperature 50℃, the ratio of liquid and solid, 10:1(mL:g), extraction time 3h.The extracts of Aspidistra elatior Blume has wide Antibacterial spectrum .The results showed that the extracts of Escherichia coli leaves had the antimicrobial actives .% 采用滤纸片法,以抑菌圈直径为评价标准,以金黄色葡萄球菌和大肠杆菌为指标菌,分别利用水以及一定百分数的乙醇提取箬叶,考察不同箬叶提取液抑菌效果.采用响应面设计优化试验得出水提取箬叶最佳提取工艺条件为:提取温度50℃,液固比10:1(mL:g),提取时间3 h,结果表明:此提取条件下箬叶提取液对大肠杆菌和金黄色葡萄球菌,具有较好的抑菌效果.
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年份:
2013
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