Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I.
摘要：
The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.
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关键词：
Apoptosis Caspases Protein-Serine-Threonine Kinases Recombinant Fusion Proteins 脱噬作用 Caspase类 蛋白质丝氨酸苏氨酸激酶
DOI：
10.1038/35070009
被引量：
年份：
2001
Nature
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Nature
Wiley
掌桥科研
万方医学
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