Transfection of DNA into adherent cells by DEAE-dextran/DMSO method increases drastically if the cells are removed from surface and treated in suspension.
摘要：
Octyl silica reversed-phase material was used as a support for dynamically coated anion surfactant, octyl sulphate, acting as an ion-pairing agent for retention of biogenic amines. In this system the influence of counterion type and concentration on retention behaviour of the analytes was studied. The strongest competitive effect of all counterions studied /Li, Na, K, NH4/ exerted potassuim and ammonium. However, the effect of potassium was lower than expected according to known value of relative selectivity. The accompanying anion and pH in range of 3,6 to 5,5 did not influence the retention noticeable. Regarding determination of catecholamines in urine after a rapid alumina cleanup, a simple ammonium acetate mobile phase of pH 5,4 gave best results with respect to separation of amines from interfering substances present in the sample.
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关键词：
Animals Tumor Cells, Cultured Neuroblastoma Dextrans Dimethyl Sulfoxide DNA DEAE-Dextran Transfection Cell Adhesion Suspensions
DOI：
10.1093/nar/17.12.4902
被引量：
年份：
1989
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