In Vitro Oxidation of Indoleacetic Acid by Soluble Auxin-Oxidases and Peroxidases from Maize Roots
摘要：
Soluble auxin-oxidases were extracted from Zea mays L. cv LG11 apical root segments and partially separated from peroxidases (EC 1.11.1.7) by size-exclusion chromatography. Auxin-oxidases were resolved into one main peak corresponding to a molecular mass of 32.5 kilodaltons and a minor peak at 54.5 kilodaltons. Peroxidases were separated into at least four peaks, with molecular masses from 32.5 to 78 kilodaltons. In vitro activity of indoleacetic acid-oxidases was dependent on the presence of MnCl2and p-coumaric acid. Compound(s) present in the crude extract and several synthetic auxin transport inhibitors (including 2,3,5-triiodobenzoic acid and N-1-naphthylphthalamic acid) inhibited auxin-oxidase activity, but had no effect on peroxidases. The products resulting from the in vitro enzymatic oxidation of [3H] indoleacetic acid were separated by HPLC and the major metabolite was found to cochromatograph with indol-3yl-methanol.
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关键词：
auxins enzymes growth regulators IAA indole-3-acetaldehyde oxidase interactions Maize metabolism NAA oxidoreductases
DOI：
10.1104/pp.94.2.485
被引量：
年份：
1990
Wiley
academic.oup.com
掌桥科研
万方医学
dx.doi.org
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