摘要：

sequence of human OPN. We found that 2K1 mono- clonal antibody could abrogate the interaction between human OPN and α9β1 integrin (27). Al- though the classical αv integrin binding sequence GRGDS and thrombin cleavage sequence YGLRS are well conserved in various species, the cryptic epitope -- SVVYGLR sequence in human OPN -- is replaced by SLAYGLR in rat and mouse OPN (16, 28). One possible approach to test the pivotal role of this cryp- tic epitope in a murine model of RA is to raise the specific antibody recognizing SLAYGLR. Thus, we have obtained the specific antisera (M5 Ab) reacting to SLAYGLR peptide. We examined the effect of M5 Ab in the murine arthritis model induced by a mix- ture of four anti-type II collagen monoclonal anti- bodies and LPS. We found that M5 Ab reacting with the SLAYGLR sequence exposed by thrombin cleav- age of murine OPN significantly suppressed the development of arthritis in mice.

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