摘要：

Lymphocyte interactions with high endothelial venules () are important to the in vivo migration of normal and neoplastic lymphocyte populations. We have previously described an 85- to 95-kDa lymphocyte surface (s) defined by mAb -1, that is involved in the recognition of by lymphocytes: antibodies against distinct of the -1 Ag differentially inhibit lymphocyte to lymph node, mucosal, or synovial . Here we characterize further the -1-defined . No well defined differences were observed between the -1 Ag immunoprecipitated from PBL and from mucosa- vs lymph -specific cell lines. The Ag is an acidic (isoelectric point = 4.2) sulfated molecule bearing both O-linked and (3,4) N-linked side chains. A subset of the -1-immunoprecipitated species is modified by covalent linkage to , yielding a Mr of approximately 180 to 200 kDa. Pulse-chase labeling reveals a major precursor of 76 kDa that appears to be processed either to the 85- to 95-kDa form or, by addition of , to a 180- to 200-kDa form. The potential role of these structural modifications, and particularly of , in the function of the putative molecules is discussed.

展开