Transcription of mouse kappa chain genes: implications for allelic exclusion.

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作者：

RP Perry，DE Kelley，C Coleclough，JG Seidman，P Leder，S Tonegawa，G Matthyssens，M Weigert

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摘要：

The nuclear RNA from a large variety of κ -producing plasmacytomas was size fractionated and analyzed with a series of cloned probes representing sequences encoding variable (V), joining (J), and constant (C) regions and selected intervening sequences. All of the plasmacytomas produce a nuclear RNA component that contains Vκand Cκsequences as well as the intervening sequence between Jκand Cκ, and that has a distinctive size depending on which of the four Jκsegments is expressed (i.e., is present in the secreted κ chain). These RNAs are the precursors of κ mRNAs, which are transcribed from productively rearranged Cκgenes. Half of the plasmacytomas examined produce, in addition to a κ mRNA precursor, a discrete component of about 8.4 kilobases that contains Cκand upstream flanking sequences but lacks the expressed V region sequence. The ability to produce this component is always associated with the persistence in the tumor genome of an unrearranged (germline) Jκ-Cκregion. In tumors rearranged at both κ loci the nonproductive allele is either transcriptionally silent or, in a minority of cases, transcribed and processed into a ``fragment'' mRNA lacking V region sequences. These results reveal that allelic exclusion can be effected at several levels of gene expression. They also provide some insight into the relative contributions of the V and C gene elements to this expression.

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关键词：

Biochemistry Nuclear RNA mRNA Precursors Gene Rearrangements Plasmacytoma Cells

DOI：

10.1073/pnas.77.4.1937

被引量：

694

年份：

1980