Radiation-induced cell cycle arrest compromised by p21 deficiency

来自 EBSCO

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阅读量：

202

作者：

J Brugarolas，C Chandrasekaran，JI Gordon，D Beach，T Jacks，GJ Hannon

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摘要：

THE protein p21 is a dual inhibitor of cyclin-dependent kinases 1á¤-3 and proliferating-cell nuclear antigen (PCNA) 4 , both of which are required for passage through the cell cycle. The p21 gene is under the transcriptional control of p53 (ref. 5), suggesting that p21 might promote p53-dependent cell cycle arrest or apoptosis. p21 has also been implicated in cell senescence 6 and in cell-cycle withdrawal upon terminal differentiation 7á¤-9 . Here we investigate the role of p21 in these processes using chimaeric mice composed partly of p21 -/- and partly of p21 +/+ cells. Immunohistochemical studies of the p21 +/+ and p21 -/- components of adult small intestine indicated that deletion of p21 had no detectable effect on the migration-associated differentiation of the four principal intestinal epithelial cell lineages or on p53-dependent apoptosis following irradiation. However, p21 -/- mouse embryo fibroblasts are impaired in their ability to undergo Gl arrest following DNA damage.

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关键词：

Cell Cycle Cyclins Cip1-protein Cyclin-Dependent Kinases Enzyme Inhibitors 细胞周期细胞周期蛋白类

DOI：

10.1038/377552a0

被引量：

3613

年份：

1995