Effects of TGF-βs on the growth, collagen synthesis and collagen lattice contraction of human dental pulp fibroblasts in vitro
摘要：
Transforming growth factor-β (TGF-β) is important in regulating the repair and regeneration of damaged dental pulp. For further elucidating the roles of different isoforms of TGF-β in the healing and inflammatory processes of human dental pulp, we found that TGF-β1, TGF-β2 and TGF-β3 inhibited the growth of two human dental pulp cell strains in vitro by 19–29, 18–25 and 23–26%, respectively, at a concentration of 0.5ng/ml. TGF-β also differentially stimulated the collagen synthesis of pulp cells. Collagen synthesis increased by 1ng/ml of TGF-β1 and TGF-β2 by 42 and 51%, respectively. TGF-β3 (0.1–1ng/ml) lacked of stimulatory effect on collagen synthesis of pulp cells. Pulp cells have the intrinsic capacity to contract collagen lattice, leading to decreasing of lattice diameter. An 8h exposure to TGF-β1 and TGF-β2 enhanced the pulp cell-populated collagen lattice contraction at concentrations ranging from 0.2 to 3ng/ml. At similar concentrations, TGF-β3 lacked of this stimulatory effect. When collagen lattice were detached after 24h of exposure, TGF-β1 and TGF-β2 (0.6–3ng/ml) induced the pulp cells-populated collagen lattice contraction within 4–8h of gel detachment. These results indicate that TGF-β-induced collagen lattice contraction is a late cellular event. These in vitro results indicate that effects of TGF-β isoforms on the growth, collagen synthesis and collagen lattice contraction of pulp cells may play crucial roles in the pathobiological processes of dental pulp.
展开
DOI：
10.1016/j.archoralbio.2004.10.005
被引量：
年份：
2005
通过文献互助平台发起求助，成功后即可免费获取论文全文。
相似文献
参考文献
引证文献
辅助模式
引用
文献可以批量引用啦~
欢迎点我试用！
