A magnesium ion core at the heart of a ribozyme domain
摘要：
Large ribozymes require divalent metal ions to fold. We show here that the tertiary structure of the Tetrahymena group I intron P4-P6 domain nucleates around a magnesium ion core. In the domain crystal structure, five magnesium ions bind in a three-helix junction at the centre of the molecule. Single atom changes in any one of four magnesium sites in this three-helix junction destroy folding of the entire 160-nucleotide P4-P6 domain. The magnesium ion core may be the RNA counterpart to the protein hydrophobic core, burying parts of the RNA molecule in the native structure.
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关键词：
Animals Phosphates Magnesium RNA, Catalytic Crystallography, X-Ray Nucleic Acid Conformation Introns Binding Sites Models, Molecular Tetrahymena
DOI：
10.1038/nsb0797-553
被引量：
年份：
1997
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