摘要：

The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography(HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole(NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separatedby an ODS column and then introduced to a sample loop for chiral separation by two tandemCHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in theserum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ±2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p<0.05),suggesting that serum L-3HB may be used as an indicator to determine the level of renaldamage.

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