Reiteration frequency of immunoglobulin light chain genes: further evidence for somatic generation of antibody diversity

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摘要：

Methods have been developed for preparing mouse immunoglobulin light chain mRNA of better than 90% purity. Hybridization of both λ and κ mRNAs to excess liver DNA yielded results compatible with gene reiteration frequencies of two to three. There was no evidence of hybridization of these highly purified mRNAs to reiterated DNA, and, in fact, the kinetics of hybridization were very similar to that of purified globin mRNA. Purified λ mRNA from tumors producing structurally different λ chains were used in competition hybridization experiments. An unlabeled λ mRNA competed with another, labeled λ mRNA to the same extent as homologous unlabeled λ mRNA. That is, base sequence homology among λ mRNAs is so high that any λ mRNA should cross-hybridize with all germ line variable (Vλ) genes at least for those V-regions which are represented among myelomas. From amino-acid sequence data, it is argued that there are probably more than 25 different λ V regions. Hence it is concluded that the number of germ line genes is too small to account for the diversity of dλ chains. A similar conclusion is drawn for κ chains.

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关键词：

Genetics RNA· DNA hybridization λ - and κ -chain mRNAs

DOI：

10.1073/pnas.73.1.203

被引量：

151

年份：

1976