摘要：

We have previously demonstrated that rat cerebellar Type-1 astrocytes express a very active genistein sensitive Na + /Ca 2+ exchanger, which accounts for most of the total plasma membrane Ca 2+ fluxes and for the clearance of loads induced by physiological agonists. In this work, we have explored the mechanism by which the reverse Na + /Ca 2+ exchange is involved in agonist-induced Ca 2+ signaling in rat cerebellar astrocytes. Microspectrofluorometric measurements of with Fluo-3 demonstrate that the signals associated long (> 20 s) periods of reverse operation of the Na + /Ca 2+ exchange are amplified by a mechanism compatible with calcium-calcium release, while those associated with short (< 20 s) pulses are not amplified. This was confirmed by pharmacological experiments using ryanodine receptors agonist (4-chloro- m -cresol) and the endoplasmic reticulum ATPase inhibitor (thapsigargin). Confocal microscopy demonstrates a high co-localization of immunofluorescent labeled Na + /Ca 2+ exchanger and RyRs. Low (< 50 mol/L) or high (> 500 mol/L) concentrations of l -glutamate ( l -Glu) or l -aspartate causes a rise in which is completely blocked by the Na + /Ca 2+ exchange inhibitors KB-R7943 and SEA0400. The most important novel finding presented in this work is that l -Glu activates the reverse mode of the Na + /Ca 2+ exchange by inducing Na + entry through the electrogenic Na + Glu-co-transporter and not through the ionophoric l -Glu receptors, as confirmed by pharmacological experiments with specific blockers of the ionophoric l -Glu receptors and the electrogenic Glu transporter.

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